Development and validation of an assay for the quantification of glycosides using high-performance thin-layer chromatography (HPTLC)

Abstract This paper presents a simple and rapid approach to the quantification of various glycosides using high-performance thin-layer chromatography (HPTLC). Different classes glycosides, represented by genistin ononin (both monosaccharidic O-glycosides), rutin (a disaccharidic O-glycoside) luteolin-6-C-glucoside C-glycoside), were successfully separated mixture ethyl acetate‒methanol‒glacial acetic acid‒formic acid (11:1:1:1, V/V ) as mobile phase followed derivatisation with natural product–polyethylene glycol (NP–PEG) reagent. The method was validated for these in accordance guidelines International Council Harmonisation. general applicability is demonstrated analysis large number including two (i.e. rutin, naringin) commercial products.